Recombinant proteins with modified properties are the backbone of biotechnology. To be useful in industrial settings the stability of proteins at higher temperatures typically requires improvement. However, for most currently available selection systems thermostability is a property that is difficult to select, to overcome this Bioproton utilises a combination of cell-free protein expression and on-microfluidic chip single molecule analysis to test thermostability of proteins. A high-throughput version of this tool is used to develop thermostabilised versions of new and novel enzymes. Such enzymes will allow manufacturing of new generation of functionalised feedstock.